"Sequencing a Genome, part VI: Chimeras are not just funny-looking animals".
"Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons". ^ a b Haas BJ, Gevers D, Earl AM, Feldgarden M, Ward DV, Giannoukos G, et al.Archived from the original (PDF) on 23 February 2012. "EST Assembly for the Creation of Oligonucleotide Probe Targets" (PDF). "Tentative mapping of transcription-induced interchromosomal interaction using chimeric EST and mRNA data". ^ Unneberg P, Claverie JM (February 2007).Boston, MA: Springer Science+Business Media, LLC. Handbook of Neurochemistry and Molecular Neurobiology Neural Membranes and Transport. Researchers have created receptor chimeras in their studies of Oncostatin M.CYP2C17 was thought to be a human gene, but ".is now considered an artefact based on a chimera of CYP2C18 and CYP2C19."."The first mRNA transcript isolated for." the human gene C2orf3 ".was part of an artificial chimera.".CHECK_CHIMERA of the Ribosomal Database Project.Some methods have been devised to detect chimeras, like: The latter is known to occur with sequencing of electrophoresis gels. a physical DNA sequence in a sample) or produced due to misreading the sample. a string of letters in a file that can be read as a DNA sequence) that originates from an actual chimera (i.e. Hence, it might be interpreted as a new species thereby inflating over diversity.Ī chimeric read is a digital DNA sequence (i.e. A chimera is a novel sequence that will most probably not match to any known organism. PCR chimeras are an important issue to take into account during metabarcoding, where DNA sequences from environmental samples are used to determine biodiversity. The aborted product anneals to the wrong template and continues to extend, thereby synthesizing a single sequence sourced from two different templates. It occurs when the extension of an amplicon is aborted, and the aborted product functions as a primer in the next PCR cycle. PCR chimera Ī chimera can also be an artifact of PCR amplification. It is estimated that approximately 1% of all transcripts in the National Center for Biotechnology Information's Unigene database contain a "chimeric sequence". It is usually considered to be a contaminant in transcript and expressed sequence tag (which results in the moniker of EST chimera) databases. Description Transcript chimera Ī chimera can occur as a single cDNA sequence originating from two transcripts.
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